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Effect of Twist1 overexpression on Bim expression. RLFs transfected with FLAG-tagged Twist1 (white bars) or control plasmids (black bars) were exposed to oxidant (12 μmol/L 4-HNE) or ER stress (2 μmol/L thapsigargin; (Thaps)) in the presence of 0.5% FBS overnight. Cell number was quantified as described in Materials and Methods (n = 250 cells/condition analyzed); ∗P < 0.0001.
