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. 2009 Dec;175(6):2351–2361. doi: 10.2353/ajpath.2009.080954

Figure 4.

Figure 4

Effect of Twist1 siRNA on cell number, proliferation, and caspase-3 activity in RLFs. A and B: RLFs, transfected with the indicated siRNA oligonucleotides, were incubated as indicated and relative cell number was determined as described in Materials and Methods. Data represent mean ± SEM, n = 6; ∗P < 0.05; ∗∗P < 0.005. C: RLFs were incubated as in A and B, and 10 μmol/L BrdU was added for the final 18 hours before immunofluorescence using a mAb against BrdU. Data represent mean ± SEM, n = 6. D: RLFs were incubated as in A and B, and caspase-3 activity was measured as described in Materials and Methods. Data represent mean ± SEM, n = 4 (serum starved) or 11 (0.5% serum); ∗P < 0.05; ∗∗P < 0.005.