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. 2009 Dec;175(6):2430–2438. doi: 10.2353/ajpath.2009.090544

Figure 4.

Figure 4

Administration of PE DHC results in increased recovery of bacterial lipids in the brains of mice with EAE. PBS, EtOH, or PE DHC-injected mice (25 ng, 250 ng, or 2.5 μg) were sacrificed after day 20 post-EAE immunization. The brains of these mice were removed, extracted for phospholipids, and 3-OH isoC17:0 fatty acid quantified using negative ion chemical ionization gas chromatography-mass spectrometry as described in Materials and Methods. The average 3-OH isoC17:0 recovery (three determinations per mouse brain sample) as a function of both the treatment and final EAE score is depicted as picograms of 3-OH isoC17:0 per 0.5 mg of total brain lipid extracted. The average SEM for all brain lipid determinations was ±2.2 pg/0.5 mg total lipid.