Table 2.
Spinal Cord T Cells
| Treatment (mouse) | Days after onset of EAE | Disease grade | CD4+ in spinal cord (%) | Foxp3+ in spinal cord (%) |
|---|---|---|---|---|
| EtOH-1 | 2 | 1.0 | 49.60 | 8.31 |
| EtOH-2 | 4 | 2.8 | 55.23 | 5.71 |
| EtOH-3 | 5 | 3.3 | 49.40 | 5.57 |
| EtOH-4 | 2 | 2.7 | 44.88 | 7.52 |
| EtOH-5 | 1 | 1.0 | 32.98 | 6.01 |
| EtOH-6 | 1 | 2.0 | 15.18 | 7.06 |
| Mean = 41.21 | Mean = 6.70 | |||
| ±14.78 | ±1.11 | |||
| PE DHC-1 | 5 | 3.3 | 72.00 | 4.53 |
| PE DHC-2 | 4 | 3.5 | 38.00 | 7.52 |
| PE DHC-3 | 5 | 2.9 | 68.86 | 2.75 |
| PE DHC-4 | 4 | 3.3 | 49.54 | 3.45 |
| PE DHC-5 | 2 | 2.9 | 40.83 | 3.21 |
| PE DHC-6 | 4 | 3.0 | 46.50 | 4.27 |
| Mean = 52.62 | Mean = 4.29 | |||
| ±14.42 | ±1.72 | |||
| P value EtOH vs. PE DHC | 0.1635 | 0.0397 |
The PE DHC fraction alters the composition of cells infiltrating the spinal cords of mice with EAE. Mice were sampled from three different experiments and sacrificed 1–5 days after onset of signs of EAE. Mononuclear cells were derived from the spinal cords, stained for CD4 and Foxp3, and evaluated by flow cytometry. Percent CD4 represents the percent CD4+T cells within the total spinal cord mononuclear cells. Percent Foxp3 represents the percent Foxp3+ T cells after gating on CD4+ T cells.