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. 2009 Dec;175(6):2362–2371. doi: 10.2353/ajpath.2009.080338

Figure 1.

Figure 1

Immunostaining of Thy1+ and CD44+ (GalN-D3) cells and 5 day cultured Thy1+ (GalN-D3) cells with markers for hepatocytes, hepatic stem/progenitor cells, and stellate cells. A: Sorted Thy1+ (GalN-D3) cells were plated on a collagen-coated 4-well chamber plate and cultured for 2 days. B: Thy1+ (GalN-D3) cells were cultured for 5 days. C: Sorted CD44+ (GalN-D3) cells were plated on a collagen-coated 4-well chamber plate and cultured for 2 days. Cells were fixed with cold ethanol and then double-immunostaining was performed: Panels of Phase-contrast, Thy1 (red), albumin, CD44, CK19, desmin (green), and merged with DAPI were shown. Thy1+/albumin+, Thy1+/CD44+, and Thy1+/CK19+ cells are polygonal, whereas Thy1+/desmin+ cells are spindle. Scale bars: 50 μm (A and C); 100 μm (B).