Figure 1.

U73122, a selective PLCβ inhibitor, modulates VEGF and TNFα expression by macrophages. Mouse peritoneal macrophages from C57Bl/6J mice were pretreated for 30 minutes with U73122 (0, 0.4, 2.5, and 10 μmol/L). LPS (100 ng/ml), NECA (1 μmol/L), or LPS with NECA were then added. Conditioned media were harvested after 18 hours incubation and assayed for VEGF (left) and TNFα (right) content by enzyme-linked immunosorbent assay. Data are the mean ± SEM of triplicates from an experiment that was repeated at least three times with similar results.