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. 2009 Dec;175(6):2557–2565. doi: 10.2353/ajpath.2009.090417

Figure 2.

Figure 2

Schematic representation of tissue preparation for biochemical studies. Frozen brains (right hemisphere) were homogenized in TBS, sonicated briefly (2 × 10 s), and centrifuged at 100,000 × g for 60 minutes at 4°C to generate a TBS-soluble fraction. The TBS-insoluble pellet was sonicated in 5 M guanidine and solubilized by agitation at room temperature for 4 hours (TBS-insoluble fraction). TBS-soluble fractions were analyzed using Aβ40, Aβ42, and aggregated Aβ using Bio-Plex multiplex suspension array systems and analyzed for A11-positive oligomers by dot blot analysis. TBS-insoluble fractions were analyzed Aβ40 and Aβ42 using Bio-Plex multiplex suspension array system.