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ANP inhibits the effect of VEGF on RPE barrier function. ARPE-19 cells were grown to confluence, and TEER values were obtained in the presence or absence of VEGF (10 ng/ml) and ANP (1 μmol/L). ANP blocked VEGF-induced TEER drop. Treatment with isatin (100 μmol/L) completely inhibits ANP’s effect on VEGF-induced TEER reduction; *P < 0.05.
