Figure 6.

ANP reduces BRB breakdown in vivo. To evaluate whether ANP blocks VEGF-induced retinal vascular permeability in vivo, 5 μl of ANP (25 ng/μl) was injected concomitantly with VEGF (5 μl, 25 ng/μl) intravitreally in one eye and the same volume of vehicle (5 μl of PBS) in the contra lateral eye. In other animals, 5 μl of VEGF (25 ng/μl) was injected intravitreally in one eye and the same volume of vehicle in the other eye. BRB breakdown was evaluated with the EB technique 24 hours after intravitreal injections. Bars represent mean ± SEM; n = 9, *P < 0.01.