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Role of ANP in CNV formation. To asses whether ANP inhibits CNV formation, fundi of the mice eyes were photocoagulated and treated with intravitreal injections of ANP or vehicle as control. A: Representative micrographs of CNV lesions in the choroidal flat mounts from a control and ANP-treated animal. Bar, 100 μm. B: Quantitative analysis of CNV size. ANP treated, n = 18; vehicle control, n = 20. Bars show the average of CNV size in each group. Data represent mean ± SEM; *P = 0.013.
