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. Author manuscript; available in PMC: 2010 Jul 25.
Published in final edited form as: Adv Drug Deliv Rev. 2009 Mar 31;61(9):732–745. doi: 10.1016/j.addr.2009.03.004

Fig. 1. Schematic of non-replicating lentiviral vector for stable shRNA expression.

Fig. 1

Suitable host cells (such as 293T cells) are trasfected with a mixture of plasmids consisting of i) an shRNA expression cassette, ii) a packaging cassette and iii) a heterologous (VSV-G) viral envelope expression cassette. The generated lentivirus is then used to transduce the desired cell type for shRNA expression. Because only the vector containing the shRNA expression cassette (devoid of the viral structural genes) integrates into the host cell genome in the transduced cells, shRNA is continually expressed but infectious virus is not produced.