| Sample from step 1 | 21.0 μl |
| Sodium Pyrophosphate, 40 mM | 2.5 μl |
| AMV Reverse Trancriptase 30 u | 1.5 μl ... 22 u/μl |
| Nuclease free water | 0.0 μl |
| Total Volume | 25.0 μl |
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