| First-Strand reaction | 25.0 μl |
| Second Strand 2.5X Buffer | 40.0 μl |
| BSA, 1 mg/ml | 5.0 μl |
| DNA Polymerase I 23 u | 3.0 μl |
| RNase H 0.8 u | 0.5 μl ... 1.5 u/μl |
| Nuclease free water | 26.5 μl |
| Total Volume | 100.0 μl |
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