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. Author manuscript; available in PMC: 2010 Jan 13.
Published in final edited form as: Biochemistry. 2009 Jan 13;48(1):190–197. doi: 10.1021/bi801912z

Figure 4. Effect of Preincubation of HO-1 with CPR on the Rate of Bilirubin Formation.

Figure 4

Reconstituted systems containing CPR and DLPC (▼), HO-1 and DLPC (Δ), and HO-1, CPR, and DLPC (■) were preincubated for 2 hrs at room temperature prior to assay. Proteins not included in pre-incubation procedure were added just prior to the initiation of the reaction. The final HO-1 concentration was 0.1 µM, and bilirubin production was monitored at 468nm. Assay details are discussed in “Experimental Procedures.” Values represent the mean +/− S.E. of n=3.