Fig. 1.

Putative Culex pipiens fatty acid metabolism pathway. Each box represents a gene predicted by sequence homology to encode for the enzyme indicated. Quantitative RT-PCR (qRT-PCR) was used to measure the expression ratios of these 31 genes (1, early diapause/nondiapause; 2, late diapause/nondiapause; 3, diapause termination/nondiapause). TAG, triacylglyceride; PUFA, polyunsaturated fatty acid; FAD, fatty acid desaturase; ACC, acetyl-coA carboxylase; FAS, fatty acid synthase; NHRs, nuclear hormone receptors; FABP, fatty acid binding protein; 28S, 28S large-subunit ribosomal RNA; RpL19, ribosomal protein large subunit 19 (loading controls). Data are presented as means of 3 independent samples. n.s., Not significant.