Table 3.
Summary of identified HDAC8 peptides modified with probe 3:BT 4 adduct.
| Entry No. | Peptide | Sequence (AA#s) | [M+H]+ | m/z | Charge state (z) | # of Mods | Mass tolerance ppm |
|---|---|---|---|---|---|---|---|
| 1 | IPKRASM*VHSLIEAYALHKQM*Ra,b | 034-055 | 3330.72571 | 1109.57524 | 3 | 1 | 5 |
| 2 | RASMVHSLIEAYALHKQM*RIVKPK | 037-060 | 3541.88969 | 886.22242 | 4 | 1 | 10 |
| 3 | ASM*VHSLIEAYALHKQMRIVKPK | 038-060 | 4105.10938 | 1367.70313 | 3 | 2 | 5 |
| 4 | ASM*VHSLIEAYALHKQMRIVKPK | 038-060 | 4105.10938 | 1367.70313 | 3 | 2 | 5 |
| 5 | QM*RIVKPK. | 053-060 | 1734.93338 | 867.96669 | 2 | 1 | 10 |
| 6 | VAINWSGGWHHAKK | 133-145 | 2901.37544 | 1450.18772 | 2 | 2 | 5 |
| 7 | DEASGFCYLNDAVLGILRLRRKFER | 147-171 | 3660.86731 | 609.31122 | 6 | 1 | 5 |
| 8 | GRYYSVNVPIQDGIQDEK | 222-239 | 2800.35061 | 934.11687 | 3 | 1 | 10 |
| 9 | YYQICESVLKEVYQAFNPK | 240-258 | 3041.46828 | 1521.23414 | 2 | 1 | 10 |
| 10 | AVVLQLGADTIAGDPMCSFNMTPVGIGK | 259-286 | 4244.03997 | 1060.25999 | 4 | 2 | 5 |
| 11 | AVVLQLGADTIAGDPMCSFNMTPVGIGK | 259-286 | 4244.03997 | 849.60799 | 5 | 2 | 10 |
a
* indicates an oxidized methionine
b
The underlined residues correspond to the region of the HDAC8 protein where modification by probe 3 occurred.