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. Author manuscript; available in PMC: 2009 Dec 7.
Published in final edited form as: Immunity. 1999 Aug;11(2):183–190. doi: 10.1016/s1074-7613(00)80093-x

Figure 2. Expression of ss-Peptide Transgenes.

Figure 2

(A) Expression of H-2Kb is not upregulated in TAP° mice expressing ss-peptide transgenes. Expression levels of the MHC class I molecule Kb were compared on splenocytes from ss-OVApTAP° (thin black line), ss-R4TAP° (dashed line), ss-VSVTAP° (dotted line), TAP° (bold gray line), and B6 (bold black line) mice.

(B) ss-peptide transgenic APC stimulate proliferation of peptide-specific CTL. Filled symbols represent ss-peptide transgene positive and open symbols represent control transgene negative APC. R4-specific CTL were incubated with ss-R4TAP° (filled triangles) and control TAP° (open triangles) splenocytes (left panel). VSV-specific CTL were incubated with ss-VSV (filled circles) and control B6 (open circles) splenocytes (right panel). Peptide-specific CTL (5 × 104) were cultured with increasing numbers of irradiated APC for 72 hr and were pulsed with [3H]thymidine for 18 hr.