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. Author manuscript; available in PMC: 2009 Dec 7.
Published in final edited form as: Immunity. 1999 Aug;11(2):183–190. doi: 10.1016/s1074-7613(00)80093-x

Figure 4. Exogenous R4 and ss-R4 Transgene-Expressing Cells Do Not Stimulate Proliferation or CTL Targeting by OT-1 CD8+ T cells.

Figure 4

(A) OT-1 splenocytes were cultured with OVAp (circles), R4 (diamonds), or VSV (squares) peptides and irradiated B6 splenocytes and were pulsed with [3H]thymidine for 6 hr after 36 hr of culture.

(B) OT-1 CTL were used at 10:1 effector to target ratio with OVAp (circles), R4 (diamonds), or VSV (squares) peptide coated and EL4 target cells.

(C) OT-1 splenocytes were cultured with increasing numbers of irradiated splenocytes from the following mice: ss-OVATAP° (circles), B6 (triangles), ss-R4TAP° line 1 (open diamonds), ss-R4TAP° line 2 (filled diamonds), and ss-VSVTAP° (squares). The assay was pulsed with [3H]thymidine for 18 hr after 36 hr of culture.

(D) OT-1 CTL were used at the indicated effector to target ratio with 51Cr-labeled Con A blasts as targets made from the following mice: ss-OVATAP° (circles), TAP° (triangles), ss-R4TAP° line 1 (open diamonds), and ss-VSVTAP° (squares).