Fig. 5.

The kinetics of OA transfer from MBCD to lipid vesicles (SUV and LUV) measured with pyranine. A suspension of lipid vesicles containing entrapped pyranine (100 μM egg-PC) was rapidly mixed with increasing amounts of OA:MBCD complex at a fixed molar ratio of 1 OA:6 MBCD (A, SUV; C, LUV). The final concentrations of OA:MBCD complexes were 3 μM OA:18 μM MBCD, 6 μM OA:36 μM MBCD, and 12 μM OA:72 μM MBCD. As controls, the same lipid vesicle preparations were mixed with unbound OA (3 μM, B; 6 μM, D). Lipid vesicles were also mixed with only buffer as mixing control. Each fluorescence trace is the average of 5 to 10 measurements and represents one experiment.