Fig. 5.

Expression of apoC-III increased secretion as VLDL containing [³⁵S]apoC-III and [³H]TAG. A: Control (neo) or apoC-III-expressing cells (C3) were labeled with either [³⁵S]methionine/cysteine for 3 h or [³H]glycerol for 4 h in DMEM containing 20% serum + 0.4 mM oleate. The conditioned media were fractionated by rate flotation ultracentrifugation, and metabolic labeled proteins and lipids were recovered from each fraction. A: Distribution of [³⁵S]apoC-III among the fractionated lipoproteins. B: Distribution of [³H]TAG (top panel) and [³H]PC (bottom panel). The experiment was repeated, and similar results were obtained.