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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Gastroenterology. 2009 Jul 24;137(6):2084–2095.e3. doi: 10.1053/j.gastro.2009.07.048

Figure 5. TRPA1 mediates the bradykinin-induced mechanical hypersensitivity of splanchnic colonic afferents.

Figure 5

A. TRPA1+/+ splanchnic colonic serosal afferent responding to bradykinin (1μM; for 2mins). Note increased mechanosensory response to a 2000mg vfh after the chemosensory response to bradykinin.

B. TRPA1−/− splanchnic serosal afferent responding to bradykinin (1μM; for 2mins). Note mechanosensory response is unchanged after bradykinin.

C. i) TRPA1+/+ serosal fibers that responded to bradykinin subsequently displayed mechanical hypersensitivity (*P<0.05, paired t-test). This effect was not observed in the corresponding TRPA1−/− serosal afferents that responded to bradykinin. ii) Mechanical hypersensitivity after bradykinin application only occurred in TRPA1+/+ afferents that responded to bradykinin and was not observed in unresponsive fibers (NS, P>0.05, paired t-test).

D. The chemosensory response bradykinin was similar in TRPA1+/+ and −/− fibers (NS, P>0.05, t-test).

E. The proportion of serosal afferents responding to bradykinin was similar in TRPA1+/+ and −/− mice (NS, P>0.05, Fisher’s exact test).