Table 1.
Multiplex single-cell nested RT-PCR analysis demonstrates the heterogeneity of islet hormone genes expression in single beta cells from adult, P1 neonatal and E15.5 embryonic MIP-GFP mice: gene expression at each developmental stage
| Developmental stage | Expression of gene, % (n)a | Total sorted cells (n) | |||||
|---|---|---|---|---|---|---|---|
| InsI | InsII | Gcg | Sst | Ppy | Hprt1 | ||
| E15.5 | 100 (70) | 100 (70) | 13 (9) | 13 (9) | 62 (43) | 100 (70) | (70) |
| P1 | 100 (147) | 100 (147) | 10 (15) | 12 (18) | 80 (117) | 100 (147) | (147) |
| Adult | 100 (476) | 100 (476) | 11 (53) | 29 (138) | 29 (137) | 100 (476) | (476) |
a% is percentage of cells expressing gene indicated, with or without the other genes listed vs total sorted cells at each developmental stage; n is number of cells