. 2009 Oct 23;53(1):128–138. doi: 10.1007/s00125-009-1570-x

Table 3.

Multiplex single-cell nested RT-PCR analysis of mRNA expression in single GFP⁺ cells from adult, P1 neonatal and E15.5 embryonic MIP-GFP mice: gene expression pattern at each developmental stage

Expression pattern	Gene					Developmental stage
Expression pattern	InsI	InsII	Pdx1	Neurod1	Hprt1	E15.5, % (n)^a	P1, % (n)^a	Adult, % (n)^a
A	+	+	+	+	+	100 (70)	100 (147)	100 (148)
B	−	−	−	−	+	0 (0)	0 (0)	0 (0)
Total						100 (70)	100 (147)	100 (148)

^a% is percentage of cells showing the indicated gene expression pattern vs total sorted cells; n is number of cells