Table 4.

Multiplex single-cell nested RT-PCR analysis for genes listed in single GFP⁺ cells from adult, P1 neonate and E15.5 embryonic MIP-GFP mice: gene expression at each developmental stage

Developmental stage	Expression of gene, % (n)a				Total sorted cells (n)
	InsI	Neurog3	Pax4	Hprt1
E15.5	100 (70)	11 (8)	53 (37)	100 (70)	70
P1	100 (89)	0 (0)	1 (1)	100 (89)	89
Adult	100 (77)	0 (0)	0 (0)	100 (77)	77

^a% is percentage of cells expressing gene indicated, with or without the other genes listed, vs total sorted cells at each developmental stage; n is number of cells