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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Arch Biochem Biophys. 2009 Oct 9;492(1-2):29–39. doi: 10.1016/j.abb.2009.10.001

Table 2.

Functional and Molecular Properties for FRET Variants1

Parameter EGK E92C EGK E121C
Initial-velocity
Vo, mM s−1 15±1 20±1
KATPo, µM 12±3 10±2
IIAGlc inhibition
K0.5, µM 3±0.5 6±0.6
W 0.09±0.02 0.09±0.02
FBP inhibition
K0.5, µM 390±15 640±50
W 0.01±0.01 0.05±0.03
nH 1.4±0.1 1.7±0.1
S20,w
Native 11.2±0.1 11.1±0.1
Fluorescein-labeled 11.1±0.1 11.0±0.1
Dansyl-labeled 11.1±0.1 11.0±0.1
Peptide positions 92–95  92–106 118–125
Peptide mass, predicted 831.2   2232.9 1377.5
Peptide mass, observed 831.3   2232.9 1377.4
1

Parameters were estimated from fits of functional data to the respective equations as described under Materials and Methods.