Fig. 6.
Traces 1 through 5, τ-integrated three-pulse ESEEM spectra (cosine FT) of wt hpH cSO for the samples incubated for 2 min in H2 17O buffer, obtained at Bo=1071.8 mT (gZ), 1079.5 mT, 1083.4 mT (gY), 1086 mT and 1088.6 mT (gX), respectively. Before the FT, the experimental ESEEM traces were extrapolated into the dead time using FTS algorithm.51 Experimental conditions: νmw = 29.810 GHz; mw pulses, 3 × 13 ns; temperature, 21 K. Diamonds and triangles indicate the ΔmI = 1 fundamental lines of equatorial 17O (from the OH ligand) and unidentified weakly coupled 17O, respectively. The higher-frequency lines belong to ΔmI = 2 and ΔmI = 3 fundamental lines of equatorial 17O.