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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Biochemistry. 2009 Dec 1;48(47):11178–11184. doi: 10.1021/bi901577d

Table 1. Kinetic and thermodynamic parameters for RF1 binding.

Codon KD (μM) kon (μM-1 s-1) koff (s-1)a krelease (s-1)
UAA < 0.0035 34.4 < 0.1 9.0 × 10-2 ± 0.1 × 10-2
CAA 15.4 ± 4.6 22.6 348 9.9 × 10-4 ± 0.9 × 10-4
UUU 1.6 ± 0.3 15.8 25.3 9.4 × 10-5 ± 0.4 × 10-5
CUC 6.5 ± 3.1 19.6 127.4 6.6 × 10-5 ± 1.4 × 10-5
a

, koff was calculated from KD and kon.