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Ionic strength dependence of the binding constant KA (■) and the second order rate constant k2 (▲) for native RCs. The value of kon is equal to that of k2 assuming a diffusion limited reaction. The ratio of the slopes of log kon and log KA vs I1/2 gives a value of α=0.60 ± 0.05 in good agreement with the value of α=0.59 ± 0.05 obtained from the fitting of the ionic strength dependence of k2 in native and YA L162 RCs.
