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. Author manuscript; available in PMC: 2011 Jan 15.
Published in final edited form as: Int J Pharm. 2009 Sep 25;384(1-2):15–23. doi: 10.1016/j.ijpharm.2009.09.033

Fig. 7. Effect of inhibitors on the formation of 4-nitrophenyl sulfate in BeWo cells.

Fig. 7

BeWo cytosolic homogenate was incubated with 1μM or 50μM 4-nitrophenol and 100μM 3′-phosphoadenosine-5′-phosphosulfate (PAPS) for 2 hours at 37°C in the absence and presence of different efflux transporter inhibitors (MK-571 inhibitors: MK-571, indomethacin, leukotriene C4; BCRP inhibitors: Fumitremorgin C, chrysin). The reaction mixture was then analyzed for 4-nitrophenyl sulfate. 4-nitrophenyl sulfate formed in the presence of inhibitors was normalized to the amount produced in the vehicle-treated controls. Solid and open bars indicate that lysate was incubated with 1μM and 50μM 4-nitrophenol respectively. Data has been plotted as mean ± S.E.M. * indicates p < 0.05.