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. Author manuscript; available in PMC: 2010 Sep 25.
Published in final edited form as: Neurosci Lett. 2009 Jun 17;461(3):302–305. doi: 10.1016/j.neulet.2009.06.007

Figure 3.

Figure 3

Blood contamination of brain does not affect measurement of rat cerebral heme-a. Rats were perfused with heparinized saline, followed by saline alone. Effectiveness of cerebral perfusion was confirmed by visual examination of dissected brain for residual blood. Hemes were extracted from forebrain of perfused (P) and non-perfused (NP) rats. Heme-a and heme-b were separated by HPLC and quantified as described in Materials and Methods. Heme-a and heme-b are expressed as nmol/g brain wet weight (mean ± S.D., n=3).