Fig. 3.

Secretable grp170 promotes a systemic antitumor response. Mice (n = 5) were inoculated with parental C2, C2-neo or C2-sgrp170 tumor cells in the left flank. At the same time, 2 × 10⁶ parental C2 tumor cells were injected on contralateral side (i.e., right flank). Tumor progression was followed by measuring perpendicular tumor diameters (*P < 0.01, C2.sgrp170 vs. C2.neo). Representative results of three separate experiments are shown. b Inoculation of grp170-secreting C2 cells does not induce a humoral response against grp170. Sera were collected from tumor-bearing mice, and subjected to ELISA assays to measure grp170-specific IgG. Grp170-specific IgG was used as a positive control. Results represent two independent experiments