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. 2009 Nov 20;10(10):4257–4266. doi: 10.3390/ijms10104257

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© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 2. — Effects of 8-OHDe on cell viability, melanin content, and cellular tyrosinase activity of mouse B16 melanoma cells. The cells were cultured in 6-well plates and incubated with tested agents for 3 d. Cell viability, cellular tyrosinase activity, and melanin content were assayed as described in the Experimental section. Bars represent the means ± S.D. of three independent experiments. Significant differences were determined by Student’s t-test; * p < 0.001; ** p < 0.0001 compared to control.