Table 2.

Concentration of focal adhesion, cytoskeletal and contractile proteins in rat aortic smooth muscle cells in 7-day-old cultures on pristine PE (Pristine), PE activated with plasma (Plasma), PE grafted with glycine (Gly), polyethylene glycol (PEG), bovine serum albumin (BSA), colloidal carbon particles (C) or BSA with C (BSA + C), and tissue culture polystyrene dish (PS dish).

Protein/PE modification	Talin	Vinculin	Paxillin	α-Actinin	β-Actin	α-Actin	SM1 SM2 myosins
Pristine	100 ± 9	100 ± 14	100 ± 28	100 ± 15	100 ± 8	100 ± 4	100 ± 16
Plasma	134 ± 12	154 ± 17	98 ± 2	129 ± 28	77 ± 10	91 ± 10	69 ± 18
Gly	109 ± 12	140 ± 18	136 ± 23	50 ± 8$	68 ± 3	119 ± 6C, *	78 ± 15
PEG	202 ± 16#, $	123 ± 8	152 ± 30	52 ± 10$	107 ± 8*	123 ± 6$, C, *	128 ± 14
BSA	127 ± 10	151 ± 13	143 ± 27	37 ± 9$	95 ± 7*	115 ± 7	73 ± 17
C	112 ± 4	133 ± 9	104 ± 15	89 ± 8	111 ± 17*	87 ± 3	87 ± 16
BSA + C	201 ± 14#, *	185 ± 14#, *	114 ± 18	72 ± 12	110 ± 12*	111 ± 7	108 ± 13
PS dish	155 ± 29	113 ± 12	175 ± 37	93 ± 7	55 ± 9	89 ± 10	103 ± 16

Measured by ELISA per mg of protein. Means ± S.E.M. from three to seven experiments, each performed in duplicate or in triplicate. Absorbance values were normalized to the values obtained in cell samples from pristine PE, i.e., given as a percentage of the values on pristine PE. ANOVA, Student-Newman-Keuls method. Statistical significance:

^{#, $, C, *}: p ≤ 0.05 compared to the value on pristine PE, PE irradiated with plasma, PE irradiated with plasma and exposed to colloidal C particles, and polystyrene culture dish, respectively.