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Table 3.

Primary antibodies used for immunofluorescence staining (Immf.) and Enzyme-Linked Immunosorbent Assay (ELISA) of markers of adhesion and phenotypic maturation of VSMCs.

Antibody against	Developed in, type	Company, Cat. No.	Dilution	Incubation
Chicken talin	Mouse, monoclonal	Sigma ^a T 3287	Immf.: 1:200 ELISA: 1:500	Immf.: Overnight, 4 °C ELISA: 60 min, RT ^c
Human vinculin	Mouse, monoclonal	Sigma ^a V9131	Immf.: 1:200 ELISA: 1:400	Immf.: Overnight, 4 °C ELISA: 60 min, RT ^c
Recombinant human paxilin	Rabbit polyclonal	Chemicon ^b P1093	Immf.: 1:200 ELISA: 1:400	Immf.: Overnight, 4 °C ELISA: 60 min, RT ^c
Chicken α-actinin	Rabbit, polyclonal	Sigma ^a A2543	Immf.: 1:200 ELISA: 1:500	Immf.: Overnight, 4 °C ELISA: 60 min, RT ^c
Synthetic peptide of α-smooth muscle actin	Mouse, monoclonal	Sigma ^a A2547	Immf.: 1:200 ELISA: 1:400	Immf.: Overnight, 4 °C ELISA: 60 min, RT ^c
Synthetic peptide of β-actin	Mouse, monoclonal	Sigma ^a A 5441	Immf.: 1:200 ELISA: 1:400	Immf.: Overnight, 4 °C ELISA: 60 min, RT ^c
Human smooth muscle myosin SM1 and SM2	Mouse, monoclonal	Sigma ^a M7786	Immf.: 1:200 ELISA: 1:500	Immf.: Overnight, 4 °C ELISA: 60 min, RT ^c

Sigma, St. Louis, MO, USA.; Czech Dealer: Sigma-Aldrich S.R.O., Prague, Czech Republic.

Chemicon International Inc., Temecula, CA, USA.; Czech dealer: Scintilla S.R.O., Jihlava, Czech Republic.

Room temperature (RT).