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Expression of DLC-1 mRNA in multiple myeloma cell lines determined by real-time RT-PCR. Gene expression was normalized to the expression of control gene GAPDH. Normal bone marrow was used as a calibrator, it's expression of DLC-1 was set at 1 and the difference from DLC-1 expression in myeloma cell lines was revealed by the 2-ΔΔCt method. Samples were divided into three subgroups according to the level of expressed DLC-1 mRNA and median of expression was determined for each subgroup.
