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. Author manuscript; available in PMC: 2010 Apr 30.
Published in final edited form as: Cell. 2009 Oct 30;139(3):560–572. doi: 10.1016/j.cell.2009.08.047

Fig. 6. EGG-4 inhibits MBK-2 kinase activity in vitro.

Fig. 6

A. 0.5, 1 and 2-fold molar excess of FLAG-tagged EGG-3, EGG-4 and EGG-4(H599A,G603A, R606A) were added to MBP:MBK-2 kinase reactions and the amount of phosphorylated MEI-1 was quantified (as in Fig. 3) after 30 minutes. Levels are expressed as ratio to that observed with MBP:MBK-2 alone (first lane).

B. Same as A, but kinase reactions were split to examine amount of tyrosine-phosphorylated MBK-2 (P-Tyr), amount of MBP:MBK-2 and amount of phosphorylated MEI-1 (P32). Note that addition of EGG-4 lowers the amount of phosphorylated MEI-1 but does not affect tyrosine phosphorylation of MBK-2.

C. Michaelis-Menten plot to compare MBK-2 enzyme kinetics in the presence (2X molar excess) or absence of FLAG:EGG-4.

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