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Fractionation of NOD from a mutant overexpressor. (A) Fractions were separated on a 8–16% SDS/PAGE gel and stained with Coomassie blue. Lanes: 1, cell-free lysate, 25 μg; 2, 33–55% ammonium sulfate dialysate, 25 μg; 3, FAD-agarose eluate concentrate, 2 μg; 4, Superdex 200 pooled concentrate, 1.2 μg; and 5, Mono Q 0.2 M NaCl eluate concentrate, 2.9 μg. (B) Superdex 200 elution profile of the NOD activity and protein. The arrow points to the elution point of the marker BSA.
