Table 7.
Rise and decay time constants of experimental macroscopic TMA or choline concentration jump current responses and of simulated macroscopic currents
| τon (ms) | τ1off (ms) | τ2off (ms) | A2/A1 (%) | n | |
|---|---|---|---|---|---|
| Choline, 10 mm | |||||
| Experiment; 5 ms | (0.8 ± 0.1 (n= 13)) | 0.76 ± 0.07 | — | — | 4 |
| Experiment; 50 ms (one component) | 1.1 ± 0.2 | — | — | 7 | |
| Experiment; 50 ms (two components) | 1.0 ± 0.1 | 30 ± 20 | 5.2 ± 0.6 | 2 | |
| Simulation Scheme 4; 5 ms | 1.3 ± 0.2 | 1.0 ± 0.2 | 60 ± 8 | 0.9 ± 0.5 | 3 |
| Simulation Scheme 4; 50 ms | 1.3 ± 0.2 | 1.0 ± 0.2 | 60 ± 8 | 10 ± 5 | 3 |
| Simulation Scheme 5; 5 ms | 0.9 ± 0.3 | 0.9 ± 0.2 | — | — | 3 |
| TMA, 10 mm | |||||
| Experiment; 5 ms | 0.30 ± 0.04 | 0.98 ± 0.07 | — | — | 10 |
| Simulation Scheme 4; 5 ms | 0.31 ± 0.04 | 0.56 ± 0.04 | 1.8 ± 0.2 | 40 ± 30 | 3 |
| Simulation Scheme 5; 5 ms | 0.54 ± 0.02 | 1.8 ± 0.3 | — | — | 3 |
Currents were simulated using the rate constants obtained from fits to Scheme 4 or Scheme 5 as responses to 5 or 50 ms 10 mm agonist square pulse. τon, on-relaxation time constant (from fits of one exponential for experiments and predictions for simulated traces). τoff, off-relaxation time constant (from fits of one or two exponentials for experiments and predictions for simulated traces). A2/A1 is ratio of current amplitudes of off-relaxation components. n, patch or set number. Time constants are given as mean ±s.d.m.