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A, time course of changes in EPSP slope of the hippocampal synapses under various conditions: CCh alone, CCh + M1 antagonist pirenzepine (2 μm), CCh + M2 antagonist AF-DX 116 (2 μm), and CCh + M1 + M2 antagonists. B, summarized EPSP reduction in both acute and sustained phases (*P < 0.05; **P < 0.01).
