TABLE I.
Target Ratio | Measured starting Ratio | Tumor cells on electrodes before Separation‡ | Tumor cells on electrodes after Separation‡ | Efficiency of purging tumor cells |
---|---|---|---|---|
1:3 | 1:2.9 | N/A | N/A | >95% |
1:103 | 1:1.1×103 | N/A | N/A | >95% |
1:104 | 1:1.2×104 | 98 | 94 | 96% |
1:104 | 1:1.8×104 | 56 | 53 | 95% |
1:105 | 1:0.9×105 | 3 | 3 | 100% |
1:3×105 | 1:2.8×105 | 1 | 1 | 100% |
: Applied voltage = 2 V rms at 50 kHz; eluate flow rate = 5 μL/min; suspension conductivity =10 mS/m.
: The in-situ counting of tumor cells in the separation column was made for the tumor to normal cell ratio higher than 1:104. Below that, the counting involved too many cells and purging efficiencies were estimated by counting residual tumor cells that contaminated the “supposed” blood cell fraction.