Table 2.

Effects of P₂ substitutions on processing of T-pαf in vivo measured by quantitative mating^*

Genotype†			Plasmids‡		(-allele)	P2	P1↓	Relative mating efficiency	± % Error§
KEX2	MFα1	MFα2	KEX2	MFα1
+	+	+	—	—		LYS	-Arg	6.4	4
Δ	Δ	Δ	+	+	-wt	LYS	-Arg	4.5	10
Δ	Δ	Δ	+	+	-100	LYS	-Arg	1.0	13
Δ	Δ	Δ	+	+	-114	ARG	-Arg	0.51	11
Δ	Δ	Δ	+	+	-116	THR	-Arg	0.13	26
Δ	Δ	Δ	+	+	-112	PRO	-Arg	0.095	8
Δ	Δ	Δ	+	+	-104	GLU	-Arg	0.088	10
Δ	Δ	Δ	+	+	-108	ILE	-Arg	0.054	7
Δ	Δ	Δ	+	+	-115	SER	-Arg	0.043	12
Δ	Δ	Δ	+	+	-101	ALA	-Arg	0.032	16
Δ	Δ	Δ	+	+	-111	ASN	-Arg	0.029	2
Δ	Δ	Δ	+	+	-117	VAL	-Arg	0.014	15
Δ	Δ	Δ	+	+	-102	CYS	-Arg	8.5 × 10−3	7
Δ	Δ	Δ	+	+	-103	ASP	-Arg	2.7 × 10−3	5
Δ	Δ	Δ	+	+	-113	GLN	-Arg	2.4 × 10−3	14
Δ	Δ	Δ	+	+	-106	GLY	-Arg	2.3 × 10−3	4
Δ	Δ	Δ	+	+	-107	HIS	-Arg	7.2 × 10−4	1
Δ	Δ	Δ	+	+	-110	MET	-Arg	6.8 × 10−4	14
Δ	Δ	Δ	+	+	-109	LEU	-Arg	4.3 × 10−4	17
Δ	Δ	Δ	+	+	-119	TYR	-Arg	1.7 × 10−4	4
Δ	Δ	Δ	+	+	-105	PHE	-Arg	6.3 × 10−6	7
Δ	Δ	Δ	+	+	-118	TRP	-Arg	<1.0 × 10−6	NA
Δ	Δ	Δ	+	+	-120	amb	-Arg	<1.0 × 10−6	NA
Δ	Δ	Δ	+	−		- - - - - - - - - -		<1.0 × 10−6	NA
Δ	Δ	Δ	−	+	-100	LYS	-Arg	<1.0 × 10−6	NA

^*Assays were performed three to eight times for each amino acid substitution at P₂ but only twice for nonmating controls (e.g., amber codon at P₂ or complete absence of KEX2 or MFα1 gene). 

^†“+ + +” strain was CRY2; “Δ Δ Δ” was ABY08. 

^‡The KEX2 plasmid was pAL5-KEX2, the MFα1-WT plasmid was pBM258-MFα1, and the rest of the plasmids were YCpG-MFα1-100 or derivatives with the indicated P₂ substitution. 

^§“% Error” is SEM expressed as a percentage.