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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Cell Metab. 2009 Nov;10(5):419–429. doi: 10.1016/j.cmet.2009.09.006

Figure 4. Insulin sensitivity in hepatocytes of obese mice correlates with reduced inflammatory signaling of Tlr4-/- hepatic macrophages.

Figure 4

CD11b liver macrophages (predominantly Kupffer cells) play an important role in regulating inflammation in the liver. Panels A and B show the increase in liver weight of HFD fed mice compared to NCD mice, and the near complete reconstitution of CD11b positive cells of the Tlr4-/- genotype in the liver of obese mice following bone marrow transplantation. Homogenized liver tissue from bone marrow transplanted mice was enriched for the macrophage specific marker CD11b and real time PCR performed to assay for wild type Tlr4 genomic DNA content. Panel C shows the fold reductions of macrophage specific and non-specific pro-inflammatory cytokines, chemoattractants, and signaling molecules of BMT-Tlr4-/- mice compared to BMT-wt mice fed a HFD. Panels D and E confirm levels of TNF-α and the chemoattractant RANTES via ELISA.