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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Cell Metab. 2009 Nov;10(5):419–429. doi: 10.1016/j.cmet.2009.09.006

Figure 6. Lentiviral vector mediated knockdown of Tlr4 in a hematopoietic stem cell gene therapy setting maintains insulin sensitivity in HFD mice.

Figure 6

Lentiviral vector driving expression of small interfering RNA (siRNA) targeted against Tlr4 in bone marrow transplanted mice of transduced and sorted hematopoietic stem cells, yields partial but significant knockdown of Tlr4 expression in peripheral blood mononuclear cells of transplanted mice as determined by real time PCR, (n=3), Panel A. Panel B shows average weights of mice fed either a high fat diet or a normal chow diet. Insulin tolerance test (ITT) was performed at 8 weeks posttransplant with LV-siTlr4 and control vector transduced bone marrow mice receiving normal chow (Panel C) or high fat diet (Panel D).