Fig. 4.

Gli2 shuttles in and out of cilia. NIH 3T3/HA-Gli2 cells infected with viruses from the LMP shRNA control vector or the same vector carrying cytoplasmic dynein 2 heavy chain (Dync2h1) shRNA were selected for puromycin resistance. Cells grown to confluency were incubated in the presence or absence of ShhN and stained for Smo or Gli2 as indicated. For greater clarity, Smo staining (green) in the Left four panels is shown as a shifted overlay with acetylated tubulin (red). Note the accumulation of both Smo and Gli2 in the primary cilium (white arrowheads) of cells infected with Dync2h1 shRNA viruses, even in the absence of ShhN, showing that impairment of the retrograde motor cytoplasmic dynein 2 causes ciliary accumulation of Smo and Gli2 in the primary cilium.