CnB1 is deleted in the PNS by Wnt1cre.(A) X-gal staining of E9.0 neural tube from a NFAT-reporter mouse line harboring LacZ under control of the DSCR1 enhancer region counterstained with nuclear fast red. Arrows, migrating neural crest cells; arrowheads, interneuron progenitors. (B) Whole-mountX-gal staining of E10.5 Rosa26; Wnt1cre mouse embryo. Arrows, sensory ganglia. (C) Immunostaining of newborn DRG and peripheral nerves at thoracic limb region by using CnB1-specific antibody. Arrows, CnB1-positive motor axons of peripheral nerves; arrowheads, ventral roots. (D and E) Immunoblots of CnB1 and NFATc3 and c4 from E13.5 DRG cocultures at day 0 (D) and day 12 (E). Heat shock protein 90 (Hsp90) is a loading control. Arrows, NFATc4 proteins. *Cross-reactive protein.