Immunoblot analysis of the parental and Ku-overexpressing Rat 1 cells with antibodies against p70 and p80 subunits of Ku. Identical amounts of protein (100 μg) from the parental Rat 1 cells (lane 1), cells overexpressing p70 subunit, R70-15 (lane 2), p80 subunit, R80-1 (lane 3), or p70 and p80 subunits together, R7080-6 (lane 4), were separated by SDS/PAGE, transferred to nitrocellulose membrane, and subjected to Western blot analysis with antibodies against p70 and p80 subunits of Ku (Santa Cruz Biotechnology).