Table 3.
Mouse bone marrow micronucleus tests of SASP and SP with kinetochore (KC) staining
| Agent | Treatment (mg/kg) | MNPCE/1000 PCE (±S.D.) |
||
|---|---|---|---|---|
| Total MN | KC− | KC+ | ||
| Vehicle control | – | 1.6 (0.19) | 1.6 (0.19) | 0.0 |
| SASP | 1875 | 4.5 (0.52) | 2.2a (0.20) | 2.3 (0.44) |
| 2721 | 5.3 (0.34) | 2.8a (0.34) | 2.5 (0.27) | |
| 3649 | 6.3 (0.68) | 2.1a (0.29) | 4.2 (0.58) | |
| SP | 2083 | 7.8 (0.88) | 4.2 (0.58) | 3.6 (0.37) |
| 2721 | 9.5 (0.42) | 3.8 (0.25) | 5.7 (0.37) | |
| 3472 | 14.2 (1.56) | 5.7 (1.33) | 8.5 (0.65) | |
| TEM (positive control) | 1.0 | 100.2 (4.94) | 96.3 (4.68) | 3.9 (0.58) |
| VCR (positive control) | 0.125 | 83.0 (2.91) | 6.7 (0.94) | 76.3 (3.00) |
Data from Witt et al. [19]. Five animals per dose group; 2000 PCEs scored per animal; vehicle control was corn oil. PCE: polychromatic erythrocyte; MNPCE: micronucleated PCE; MN: micronuclei; SASP: salicylazosulfapyridine; SP: sulfapyridine; TEM: triethylenemelamine; VCR: vincristine sulfate; KC−: kineticore negative; KC+: kineticore positive.
a
Not statistically significant, otherwise all pairwise comparisons of dosed groups to control were statistically significant at p < 0.01.