Table 4.
Effect of co-substrate concentration on the bioreduction
| 2-Propanol concentration (mM) |
Vo (μmol/min gcwm) |
Yielda (%) |
e.e.b (%) |
|---|---|---|---|
| 0 | 0.95 | 25.6 | >99 |
| 34 | 1.57 | 72.9 | >99 |
| 66 | 1.99 | 85.1 | >99 |
| 98 | 2.30 | 92.8 | >99 |
| 130 | 2.46 | 97.7 | >99 |
| 162 | 2.22 | 90.5 | >99 |
| 194 | 1.88 | 80.3 | >99 |
| 258 | 1.07 | 50.4 | >99 |
Reaction conditions: 24 mM TMSB, C4MIM·PF6/TEA-HCl buffer (100 mM, pH 5.5) volume ratio of 1/4, various concentrations of 2-propanol, 0.15 g/mL cell-loaded alginate beads, 30°C, 180 r/min.
a Maximum yield.
b Product e.e.