Table 1.
qPCR probes and primer sequences.
| Target | mRNA primer sequence 5′–3′ | Product (bp) | Reference |
|---|---|---|---|
| β-Actin | Forward: TCCTTCCTGGGCATGGAATC | 199 | U39357; NCBI 2009 |
| Reverse: GGGCGCGATGATCTTGATCT | |||
| Probe (FAM labeled): CCTTCCTTCCTGGGCATGGAATCC | |||
| KiSS-1 | Forward: CTGGTGCAGCGGGAGAAG | 57 | DQ059506; |
| Reverse: GCGCAGGCCGAAGGA | NCBI 2009 | ||
| Probe (FAM labeled): ACGTGTCCGCCTACA | |||
| ERα | Forward: CCCGGAAGACGTGAATCAGA | 66 | AY033393; |
| Reverse: GTTTGCAAGGAATGCGATGA | NCBI 2009 | ||
| Probe (FAM labeled): CAGCTGGCCACCACTGGCTGC | |||
| GPR54 | Forward: TACATCCAGCAGGTCTCGGTG | 71 | Seminara et al. 2003 |
| Reverse: ACGTACCAGCGGTCCACACT | |||
| Probe (FAM labeled): CACGTGTGCCACTCTGACCGCC |