Table 4.

The Effect of Ligand Structure on the Binding of an RNA Strand (Sequence rCUCCUCCCUCCU) Shown Are Melting Transition Temperatures (T_m (°C)) and Free Energies (−ΔG° (kcal/mol)) at pH 7.0^a

$\mathbf{\text{complex}} \left(\frac{\text{ligand}}{\text{target}}\right)$	Tm (°C) b	−ΔG°37b (kcal)	−ΔG°60b (kcal)
	58.9	15.6	8.3
	65.5	18.3	10.8
	70.5	18.0	12.3

^aConditions: 100 mM NaCl, 10 mM MgCl₂, 10 mM Na•PIPES, pH 7.0, 1.5 µM each DNA strand.

^bError in T_m values and in free energies are estimated at ±1.0 °C and ±10%, respectively.